| Course name |
Biology |
|---|
| Study-unit Code |
GP004082 |
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| Curriculum |
Biomolecolare |
|---|
| Lecturer |
Elisabetta Chiaradia |
|---|
| Lecturers |
- Elisabetta Chiaradia
- Serena Porcellati (Codocenza)
|
|---|
| Hours |
- 10 ore - Elisabetta Chiaradia
- 37 ore (Codocenza) - Serena Porcellati
|
|---|
| CFU |
6 |
|---|
| Course Regulation |
Coorte 2024 |
|---|
| Supplied |
2024/25 |
|---|
| Supplied other course regulation |
|
|---|
| Learning activities |
Affine/integrativa |
|---|
| Area |
Attività formative affini o integrative |
|---|
| Sector |
BIO/10 |
|---|
| Type of study-unit |
Opzionale (Optional) |
|---|
| Type of learning activities |
Attività formativa monodisciplinare |
|---|
| Language of instruction |
Italian |
|---|
| Contents |
Chromatographic techniques. Enzyme assays. |
|---|
| Reference texts |
Wilson K., Walker J., Biochimica e biologia molecolare, Principi e tecniche EDs. 2019, Casa Editrice Raffaello Cortina. Maccarrone M., Metodologie Biochimiche e Biomolecolari, Casa Editrice Zanichelli. Bonaccorsi di Patti M.C., Contestabile R., Di Salvo M.L., Metodologie biochimiche, Casa Editrice Zanichelli. |
|---|
| Educational objectives |
Knowledge about chromatographic methods for main biomolecules study. |
|---|
| Prerequisites |
Basic knowledge in the field of Organic Chemistry and Biochemistry. |
|---|
| Teaching methods |
Lectures. |
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| Other information |
Optional. |
|---|
| Learning verification modality |
Interview on course topics. |
|---|
| Extended program |
Lab's instruments. How to prepare solutions. Protein extraction and cellular lysis by tissue's omogeneization. Methods and techniques for protein purification: centrifugazion, fractional protein's precipitation, dialysis. Chromatographic techniques: introduction. Molecular exclusion chromatography. Ion exchange chromatography. Affinity chromatography. Partition chromatography: thin-layer chromatography. Adsorption chromatography. High-pressure liquid chromatography. Enzyme kinetics: measure of enzyme catalitic activity. Specific activity, Km and Vmax values. Enzymatic inhibition. |
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